Plot

The pregnant Bride and her groom rehearse their wedding. Bill − the Bride's former lover, the father of her child, and the leader of the Deadly Viper Assassination Squad − arrives unexpectedly and orders the Deadly Vipers to kill everyone at the wedding. Bill shoots the Bride in the head, but she survives and swears revenge.

Four years later, the Bride has already assassinated Deadly Vipers O-Ren Ishii and Vernita Green. She goes to the trailer of Bill's brother and Deadly Viper Budd, planning to ambush him. Budd has been warned by Bill of her approach; he incapacitates her with a non-lethal shotgun blast of rock salt and sedates her. He calls Elle Driver, another former Deadly Viper, and arranges to sell her the Bride's unique sword for $1 million. He seals the Bride inside a coffin and buries her alive.

Years earlier, Bill tells the young Bride of the legendary martial arts master Pai Mei and his Five Point Palm Exploding Heart Technique, a death blow that Mei refuses to teach his students; the technique that supposedly kills any opponent after they have taken five steps. Bill takes the Bride to Mei's temple for training. Mei ridicules her and makes her training a torment, but she gains his respect. In the present, the Bride uses Mei's techniques to break out of the coffin and claw her way to the surface.

Elle arrives at Budd's trailer and kills him with a black mamba hidden with the money for the sword. She calls Bill and tells him that the Bride has killed Budd and that she has killed the Bride, using the Bride's real name: Beatrix Kiddo. As Elle exits the trailer, Beatrix ambushes her and they fight. Elle, who was also taught by Mei, taunts Beatrix by revealing that she killed Mei by poisoning him in retribution for him plucking out her eye. An enraged Beatrix plucks out Elle's remaining eye and leaves her screaming in the trailer with the black mamba.

In Acuña, Mexico, Beatrix meets a retired pimp, Esteban Vihaio, who helps her find Bill. She tracks him to a hotel, and discovers that their daughter B.B. is still alive, now four years old, spending the evening with them. After she puts B.B. to bed, Bill shoots Beatrix with a dart containing truth serum and interrogates her. She recounts a mission in which she discovered she was pregnant and explains that she left the Deadly Vipers to give B.B. a better life. Bill explains that he assumed she was dead; he ordered her assassination when he discovered she was alive and engaged to a "jerk" he assumed was the father of her child. Beatrix disables Bill and strikes him with the Five Point Palm Exploding Heart Technique. Surprised that Mei taught her the technique, Bill makes his peace with her, takes five steps, and dies. Beatrix leaves with B.B. to start a new life.